However, to be able to distinguish between two objects under a microscope, a viewer must first magnify to a point at which resolution becomes relevant. Resolution depends on the distance between two distinguishable radiating points. A microscopic imaging system may have many individual components, including a lens and recording and display components. Each of these contributes to the optical resolution of the system, as will the environment in which the imaging is performed.
Real optical systems are complex, and practical difficulties often increase the distance between distinguishable point sources. At very high magnifications with transmitted light, point objects are seen as fuzzy discs surrounded by diffraction rings.
These are called Airy disks. It will be a number larger than 1. For instance a 10x objective magnifies the image ten-fold. The magnifying factor for each objective always printed on it, and the magnifying factor for each eyepiece is usually printed on it.
If the eyepiece is missing a printed magnifying factor, you can usually assume it is 10x. The total magnification for any image viewed under a compound microscope is calculated by using the formula:. So, each time you switch objectives, you change the total magnification. There is a compound microscope for each student.
Carry out the activities listed below and fill in the blanks as you do so. Objective two:. Objective three:. The theories of resolution and magnification play a major role in fields such as astronomy, astrophysics, navigation, biology and any other field that has applications of optics.
In this article, we are going to discuss what resolution and magnification are, their definitions, how the resolution and the magnification can be adjusted or changed, the applications of resolution and magnification, the similarities between resolution and magnification, and finally the difference between resolution and magnification.
Magnification is a property discussed in optics. In more common words, magnification means how many times the original image is magnified by a certain object or a method. The simplest kind of magnification is the magnification glass.
This is also known as the simple microscope. There are two methods for calculating magnification and other optical properties. Numerical aperture is a number that expresses the ability of a lens to resolve fine detail in the object being observed. Magnification is simply how much an image is enlarged.
The images below are the same small printed part with text on it. The first image was captured using a stereo microscope with a lens that has NA 0.
This image was captured at 90x.
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